Publications
2015
Nuclear pores. Architecture of the nuclear pore complex coat,
Stuwe, T., Correia A. R., Lin D. H., Paduch M., Lu V. T., Kossiakoff A. A., and Hoelz A.
, Science, 03/2015, Volume 347, Number 6226, p.1148-52, (2015)
Protein targeting. Structure of the Get3 targeting factor in complex with its membrane protein cargo,
Mateja, A., Paduch M., Chang H. Y., Szydlowska A., Kossiakoff A. A., Hegde R. S., and Keenan R. J.
, Science, 03/2015, Volume 347, Number 6226, p.1152-5, (2015)
Scalable high throughput selection from phage-displayed synthetic antibody libraries,
Miersch, S., Li Z., Hanna R., McLaughlin M. E., Hornsby M., Matsuguchi T., Paduch M., Saaf A., Wells J., Koide S., et al.
, J Vis Exp, 01/2015, Number 95, p.51492, (2015)
2014
Human-chromatin-related protein interactions identify a demethylase complex required for chromosome segregation,
Marcon, E., Ni Z., Pu S., Turinsky A. L., Trimble S. S., Olsen J. B., Silverman-Gavrila R., Silverman-Gavrila L., Phanse S., Guo H., et al.
, Cell Rep, Volume 8, Number 1, p.297-310, (2014)
Probing the functions of the paramyxovirus glycoproteins F and HN with a panel of synthetic antibodies,
Welch, B. D., Paduch M., Leser G. P., Bergman Z., Kors C. A., Paterson R. G., Jardetzky T. S., Kossiakoff A. A., and Lamb R. A.
, J Virol, Volume 88, Number 20, p.11713-25, (2014)
Structural mechanism of voltage-dependent gating in an isolated voltage-sensing domain,
Li, Q., Wanderling S., Paduch M., Medovoy D., Singharoy A., McGreevy R., Villalba-Galea C. A., Hulse R. E., Roux B., Schulten K., et al.
, Nat Struct Mol Biol, Volume 21, Number 3, p.244-52, (2014)
2013
Generating conformation-specific synthetic antibodies to trap proteins in selected functional states,
Paduch, M., Koide A., Uysal S., Rizk S. S., Koide S., and Kossiakoff A. A.
, Methods, Volume 60, Number 1, p.3-14, (2013)
Structure of active beta-arrestin-1 bound to a G-protein-coupled receptor phosphopeptide,
Shukla, A. K., Manglik A., Kruse A. C., Xiao K., Reis R. I., Tseng W. C., Staus D. P., Hilger D., Uysal S., Huang L. Y., et al.
, Nature, Volume 497, Number 7447, p.137-41, (2013)
2012
Identification of a tetratricopeptide repeat-like domain in the nicastrin subunit of gamma-secretase using synthetic antibodies,
Zhang, X., Hoey R. J., Lin G., Koide A., Leung B., Ahn K., Dolios G., Paduch M., Ikeuchi T., Wang R., et al.
, Proc Natl Acad Sci U S A, Volume 109, Number 22, p.8534-9, (2012)
Substance P derivatives as versatile tools for specific delivery of various types of biomolecular cargo,
Rizk, S. S., Misiura A., Paduch M., and Kossiakoff A. A.
, Bioconjug Chem, Volume 23, Number 1, p.42-6, (2012)
Publications
- Characterization of synthetic antigen binding fragments targeting Toc75 for the isolation of TOC in A. thaliana and P. sativum.
- Development of a universal nanobody-binding Fab module for fiducial-assisted cryo-EM studies of membrane proteins.
- Development, structure, and mechanism of synthetic antibodies that target claudin and Clostridium perfringens enterotoxin complexes.
- Engineering of a synthetic antibody fragment for structural and functional studies of K+ channels.
- Isoform- and ligand-specific modulation of the adhesion GPCR ADGRL3/Latrophilin3 by a synthetic binder.
- Quaternary structure independent folding of voltage-gated ion channel pore domain subunits.
- Structural basis for assembly and lipid-mediated gating of LRRC8A:C volume-regulated anion channels.
- Structure of an AMPK complex in an inactive, ATP-bound state.
- Structure of human NTCP reveals the basis of recognition and sodium-driven transport of bile salts into the liver.
- Structure, sequon recognition and mechanism of tryptophan C-mannosyltransferase.
- Structures of ABCB4 provide insight into phosphatidylcholine translocation.
- Structures of atypical chemokine receptor 3 reveal the basis for its promiscuity and signaling bias.
- Synthetic Antibodies Detect Distinct Cellular States of Chromosome Passenger Complex Proteins.
- Targeting a proteolytic neoepitope on CUB domain containing protein 1 (CDCP1) for RAS-driven cancers.