Selection of recombinant anti-SH3 domain antibodies by high-throughput phage display.

TitleSelection of recombinant anti-SH3 domain antibodies by high-throughput phage display.
Publication TypeJournal Article
Year of Publication2015
AuthorsHuang, Haiming, Economopoulos Nicolas O., Liu Bernard A., Uetrecht Andrea, Gu Jun, Jarvik Nick, Nadeem Vincent, Pawson Tony, Moffat Jason, Miersch Shane, and Sidhu Sachdev S.
JournalProtein Sci
Volume24
Issue11
Pagination1890-900
Date Published2015 Nov
ISSN1469-896X
KeywordsAmino Acid Sequence, Antibodies, Cell Surface Display Techniques, HEK293 Cells, High-Throughput Screening Assays, Humans, Molecular Sequence Data, Protein Engineering, Recombinant Fusion Proteins, Sequence Alignment, src Homology Domains
Abstract

<p>Antibodies are indispensable tools in biochemical research and play an expanding role as therapeutics. While hybridoma technology is the dominant method for antibody production, phage display is an emerging technology. Here, we developed and employed a high-throughput pipeline that enables selection of antibodies against hundreds of antigens in parallel. Binding selections using a phage-displayed synthetic antigen-binding fragment (Fab) library against 110 human SH3 domains yielded hundreds of Fabs targeting 58 antigens. Affinity assays demonstrated that representative Fabs bind tightly and specifically to their targets. Furthermore, we developed an efficient affinity maturation strategy adaptable to high-throughput, which increased affinity dramatically but did not compromise specificity. Finally, we tested Fabs in common cell biology applications and confirmed recognition of the full-length antigen in immunoprecipitation, immunoblotting and immunofluorescence assays. In summary, we have established a rapid and robust high-throughput methodology that can be applied to generate highly functional and renewable antibodies targeting protein domains on a proteome-wide scale.</p>

DOI10.1002/pro.2799
Alternate JournalProtein Sci.
PubMed ID26332758
PubMed Central IDPMC4622221
Grant ListMOP-93684 / / Canadian Institutes of Health Research / Canada

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