Nuclear pores. Architecture of the nuclear pore complex coat

TitleNuclear pores. Architecture of the nuclear pore complex coat
Publication TypeJournal Article
Year of Publication2015
AuthorsStuwe, T., Correia A. R., Lin D. H., Paduch M., Lu V. T., Kossiakoff A. A., and Hoelz A.
Date Published03/2015
ISSN1095-9203 (Electronic) 0036-8075 (Linking)

The nuclear pore complex (NPC) constitutes the sole gateway for bidirectional nucleocytoplasmic transport. Despite half a century of structural characterization, the architecture of the NPC remains unknown. Here we present the crystal structure of a reconstituted  400-kilodalton coat nucleoporin complex (CNC) from Saccharomyces cerevisiae at a 7.4 angstrom resolution. The crystal structure revealed a curved Y-shaped architecture and the molecular details of the coat nucleoporin interactions forming the central "triskelion" of the Y. A structural comparison of the yeast CNC with an electron microscopy reconstruction of its human counterpart suggested the evolutionary conservation of the elucidated architecture. Moreover, 32 copies of the CNC crystal structure docked readily into a cryoelectron tomographic reconstruction of the fully assembled human NPC, thereby accounting for  16 megadalton of its mass.


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