Architecture of the fungal nuclear pore inner ring complex.

TitleArchitecture of the fungal nuclear pore inner ring complex.
Publication TypeJournal Article
Year of Publication2015
AuthorsStuwe, Tobias, Bley Christopher J., Thierbach Karsten, Petrovic Stefan, Schilbach Sandra, Mayo Daniel J., Perriches Thibaud, Rundlet Emily J., Jeon Young E., Collins Leslie N., Huber Ferdinand M., Lin Daniel H., Paduch Marcin, Koide Akiko, Lu Vincent, Fischer Jessica, Hurt Ed, Koide Shohei, Kossiakoff Anthony A., and Hoelz André
JournalScience
Volume350
Issue6256
Pagination56-64
Date Published2015 Oct 02
ISSN1095-9203
KeywordsAmino Acid Sequence, Chaetomium, Fungal Proteins, Molecular Sequence Data, Nuclear Pore, Nuclear Pore Complex Proteins, Nuclear Proteins, Protein Binding, Protein Multimerization, Protein Structure, Secondary, Protein Structure, Tertiary
Abstract

<p>The nuclear pore complex (NPC) constitutes the sole gateway for bidirectional nucleocytoplasmic transport. We present the reconstitution and interdisciplinary analyses of the ~425-kilodalton inner ring complex (IRC), which forms the central transport channel and diffusion barrier of the NPC, revealing its interaction network and equimolar stoichiometry. The Nsp1•Nup49•Nup57 channel nucleoporin heterotrimer (CNT) attaches to the IRC solely through the adaptor nucleoporin Nic96. The CNT•Nic96 structure reveals that Nic96 functions as an assembly sensor that recognizes the three-dimensional architecture of the CNT, thereby mediating the incorporation of a defined CNT state into the NPC. We propose that the IRC adopts a relatively rigid scaffold that recruits the CNT to primarily form the diffusion barrier of the NPC, rather than enabling channel dilation.</p>

DOI10.1126/science.aac9176
Alternate JournalScience
PubMed ID26316600
PubMed Central IDPMC4826903
Grant ListU01-GM094588 / GM / NIGMS NIH HHS / United States
T32 GM007616 / GM / NIGMS NIH HHS / United States
R01 GM090324 / GM / NIGMS NIH HHS / United States
U01 GM094588 / GM / NIGMS NIH HHS / United States
P30 CA014599 / CA / NCI NIH HHS / United States
ACB-12002 / / PHS HHS / United States
AGM-12006 / / PHS HHS / United States
R01-GM111461 / GM / NIGMS NIH HHS / United States
U54 GM087519 / GM / NIGMS NIH HHS / United States
P30-CA014599 / CA / NCI NIH HHS / United States
U54-GM087519 / GM / NIGMS NIH HHS / United States
R01-GM090324 / GM / NIGMS NIH HHS / United States
R01 GM111461 / GM / NIGMS NIH HHS / United States

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